Super-resolution microscopy reveals coupling between mammalian centriole subdistal appendages and distal appendages
نویسندگان
چکیده
منابع مشابه
Hierarchical assembly of centriole subdistal appendages via centrosome binding proteins CCDC120 and CCDC68
In animal cells, the centrosome is the main microtubule-organizing centre where microtubules are nucleated and anchored. The centriole subdistal appendages (SDAs) are the key structures that anchor microtubules in interphase cells, but the composition and assembly mechanisms of SDAs are not well understood. Here, we reveal that centrosome-binding proteins, coiled-coil domain containing (CCDC) 1...
متن کاملArchitecture of mammalian centriole distal appendages accommodates distinct blade and matrix functional elements
Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei, 10617, Taiwan Institute of Biochemistry and Molecular Biology, National Yang Ming University, Taipei, 11221, Taiwan Cell Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA Institute of Cancer Research, London, United Kingdom Institute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, ...
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Formation of cilia, microtubule-based structures that function in propulsion and sensation, requires Kif3a, a subunit of Kinesin II essential for intraflagellar transport (IFT). We have found that, Kif3a is also required to organize centrioles. In the absence of Kif3a, the subdistal appendages of centrioles are disorganized and lack p150(Glued) and Ninein. Consequently, microtubule anchoring, c...
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The primary cilium originates from the mother centriole and participates in critical functions during organogenesis. Defects in cilia biogenesis or function lead to pleiotropic phenotypes. Mutations in centrosome-cilia gene CC2D2A result in Meckel and Joubert syndromes. Here we generate a Cc2d2a(-/-) mouse that recapitulates features of Meckel syndrome including embryonic lethality and multiorg...
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Cytotoxic T lymphocytes (CTLs) are highly effective serial killers capable of destroying virally infected and cancerous targets by polarized release from secretory lysosomes. Upon target contact, the CTL centrosome rapidly moves to the immunological synapse, focusing microtubule-directed release at this point [1-3]. Striking similarities have been noted between centrosome polarization at the sy...
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ژورنال
عنوان ژورنال: eLife
سال: 2020
ISSN: 2050-084X
DOI: 10.7554/elife.53580